Fig. 5: Estimated cell enrichments for NMF clusters in the cortex and spinal cord.
From: Machine learning predicts distinct biotypes of amyotrophic lateral sclerosis
Standard deviations (SDs) from mean based on empirical null distribution are plotted for cortex (CTX) and spinal cord (SC) samples using Expression Weighted Cell type Enrichment (EWCE). Results are plotted for both ALS patients and controls (ALSC) in the CTX (A), and SC (B). As well as the ALS patients, only NMF analysis for CTX (C) and SC (D). NMF clusters are ordered according to their pathophysiology (synaptic dysfunction, neuronal regeneration, neuronal degeneration, or control). Asterisks represent significant effects based on Bonferroni corrected p-value. The color of the bar represents whether the analysis was performed on the 250 most upregulated (orange) or downregulated (blue) features from the corresponding DEA. Only positive differences of SDs from mean are shown. Each cluster had a distinct pattern of estimated cell group-specific up- or downregulation.
