Fig. 5

Immunohistochemical NIS staining. Paraffin embedded tumor sections were immunohistochemically stained for NIS expression. Tumors from mice treated with polyplexes for dual-targeting revealed clusters of membrane-associated as well as cytoplasmatic NIS gene expression around blood vessels in the tumorous tissue. Animals that were injected with polyplexes for single EGFR- and cMET-targeting showed NIS-specific immunoreactivity as well that correlated with PET imaging data