Fig. 4: Chimeric viruses expressing the F gene of lytic strain Anhinga induces serious cell death in vitro. | Gene Therapy

Fig. 4: Chimeric viruses expressing the F gene of lytic strain Anhinga induces serious cell death in vitro.

From: RETRACTED ARTICLE: Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Fig. 4

a Syncytia promotion analysis of different cancers. Cancer cells (HepG2, MDA-MB-231, A549, and MCF7) were infected with recombinant viruses at MOI of 0.1, and photomicrographs of cell monolayers were captured at 24 h post-infection (left). Cells were counterstained with DAPI (4′,6-diamidino-2-phenylindole) for localization of nuclei (right). Representative fields of view were shown at ×100 magnification. The cancer cells incubated with normal allantoic fluid were used as control. b Cytotoxicity effects of recombinant viruses on cancer cells. The cancer cells HepG2, MDA-MB-231, A549, and MCF7 cells incubated with normal allantoic fluid were used as control. MTT method was used to detect the cell density at 490 nm. The data are the means ± SEM. of triple samples (*P < 0.05; **P < 0.01). c, d NDV induce more apoptotic cells in susceptible cells. Apoptosis cells were quantified by flow cytometry with Annexin V-FITC and PI. Representative scatter plots of PI (y-axis) versus FITC (x-axis). Values represent mean ± SEM. with three replicates. *P < 0.05, **P < 0.01, compared with control. c HepG2. d MCF7.

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