Fig. 7: Chimeric viruses expressing the F gene of lytic strain Anhinga induce more T-cell infiltration, necrotic foci, and apoptotic cells in vivo. | Gene Therapy

Fig. 7: Chimeric viruses expressing the F gene of lytic strain Anhinga induce more T-cell infiltration, necrotic foci, and apoptotic cells in vivo.

From: RETRACTED ARTICLE: Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Fig. 7

a H&E staining of H22 tumor sections (left) and 4T1 tumor sections (right) after treatment of viruses. Representative fields of view were shown at ×100 magnification and ×400 magnification. b TUNEL assay (terminal deosynucleotidy transferase-mediated dUTP nick and labeling assay) was used to detect the apoptosis of tumor cells and the results were shown in brown. The larger the brown area, the more serious the apoptosis of cancer cells was. Representative fields of view were shown at ×100 magnification. c, d Transcriptional levels of Bax and Bcl2 genes in the tumor of H22 (left) and 4T1(right) tumor-bearing mice were calculated relative to the β-actin. Control: untreated mice. Model: mice treated with allantoic fluid. *P < 0.05, **P < 0.01, compared with the Model group. #P < 0.05, ##P < 0.01, n = 3, compared with the rClone30 group.

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