Fig. 10: RDEB keratinocytes transfections with the HPAE-EB polymer and CRISPR-C7 plasmid.
From: Non-viral delivery of CRISPR–Cas9 complexes for targeted gene editing via a polymer delivery system
a Efficient transfection was evaluated by GFP expression. Cells were treated with different polymer:DNA ratios and DNA amounts (VII–XII). Furthermore, Lipofectamine 3000 was used as the commercial reagent for comparison (V, VI), untreated cells were used as a negative control (I, II) and plasmid DNA as the vector control (III, IV). Scale bar 100 µm. Representative images from six replicates of three independent experiments (n = 3). b alamarBlue™ test showed high preservation of RDEB keratinocytes viability 72 h post transfection using different HPAE-EB polymer conditions complexed to DNA, and Lipofectamine 3000 as a control. Data were collected from six replicates of three independent experiments and presented as mean ± SD (n = 3).
