Fig. 11: FACS analysis of GFP-positive RDEB keratinocytes.
From: Non-viral delivery of CRISPR–Cas9 complexes for targeted gene editing via a polymer delivery system
Gating strategy stratified cells into three groups, namely, GFP negative, GFP “dim” and GFP “bright”, based on fluorescence intensity. Optimal transfection conditions using HPAE-EB and CRISPR-C7 plasmid resulted in 11.5% RDEB keratinocytes that were highly fluorescent. Data are representative of three replicates from three independent experiments (n = 3).
