Fig. 12: PCR analysis of genomic DNA from RDEB keratinocytes treated under different transfection conditions with 0.5 µg CRISPR-C7 plasmid. | Gene Therapy

Fig. 12: PCR analysis of genomic DNA from RDEB keratinocytes treated under different transfection conditions with 0.5 µg CRISPR-C7 plasmid.

From: Non-viral delivery of CRISPR–Cas9 complexes for targeted gene editing via a polymer delivery system

Fig. 12

Upper arrow of 320 bp band indicates unedited DNA, while lower arrow of 262 bp band corresponds to DNA lacking exon 80. Maximum excision efficiency of 8.2% was estimated by densitometry analysis. Data are representative of three independent experiments (n = 3).

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