Fig. 4: Evaluation of anti-AAV5 antibody interference.
From: Ultra-sensitive AAV capsid detection by immunocapture-based qPCR following factor VIII gene transfer
Plasma samples were spiked with increasing amounts of anti-AAV5 antibodies and either left unspiked (NQC, green bars) or spiked with AAV5-FVIII-SQ at 2E+06 vg/mL (LQC, pink bars) and 2E+09 vg/mL (HQC, blue bars) and tested in iqPCR. Dotted lines represent the target performance range, based on the capsid concentrations for LQC (bottom range) and HQC (top range) measured in the absence of anti-AAV5 antibodies, and using an RE from −50 to 100%.
