Fig. 4: Quantification of neutralizing antibodies in KC50.
From: Suppression of viral rebound by a Rev-dependent lentiviral particle in SIV-infected rhesus macaques
Quantification of neutralizing antibodies was conducted using a pseudovirus-based antibody neutralization assay, which utilizes a pseudovirus carrying the envelope of the tier 1 strain SIVmac251.6 or an MLV-pseudotyped virus as a negative control (a). A pseudovirus carrying the envelope of the tier 2 strain SIVmac239CS.23 was also quantified (b). The ID50 values represent serum dilutions at which 50% neutralization occurred. For comparison, plasma viral loads in KC50 were also plotted (red line). The background signals against MLV-pseudovirus are all <20. Positive for neutralizing antibody activity is based on the criterion of ≥3× signal of the MLV control.
