Fig. 3: The effect of uPSEM⁸¹⁷ on PSAM⁴-GlyR causes alterations in some evoked action potential properties.

Whole-cell patch-clamp recording of CaMKIIα-PSAM⁴-GlyR-eGFP+ cells (green) and control GFP-only cells (Ctrl, orange). Measurements in depolarizing ramp currents of the APth (A), APamp (B), and Imin for the first AP ((C), left). Normalized values for Imin for the first AP ((C), right). The minimum current needed to trigger APs in depolarizing step currents (D). Measurements in Imin depolarizing step for APamp (E) and APahp (F). Steady-state current amplitude in 500 pA step (G). AP, action potential; Imin, minimum current to trigger an AP in step currents; APth AP threshold; APamp AP amplitude; APahp AP afterhyperpolarization amplitude. The dashed line indicates that the cell did not generate an AP during the uPSEM⁸¹⁷ effect, thereby there is no value in the analysis for that cell and the line connects the value from the baseline directly to the wash-out. CaMKIIα-PSAM⁴-GlyR-eGFP+ cells: n = 12, CaMKIIα-eGFP+ cells: n = 7. Median ± interquartile range. Wilcoxon paired test for comparison of the PSEM effect to the Baseline. *, p < 0.05; **, p < 0.01.