Fig. 7: Thermostability evaluation of the untreated and trypsin-treated AAV2 capsids.
From: Adeno-associated virus serotype 2 capsids with proteolytic cuts by trypsin remain intact and potent
The genome ejection melting curves A were based on the fluorescence intensity of SYBR Gold bound to DNA; the capsid unfolding curves B were based on intrinsic fluorescence of capsid proteins. The untreated AAV2 capsids in black trace; trypsin-treated AAV2 capsids in red trace (treated for 10 min), in green trace (treated for 30 min), and in sky blue (treated for 60 min) (n = 2, technical replicates).
