Fig. 3: High resolution histopathology illustrating axon protection and enhanced remyelination in spinal cord of EAE mice following AAV1.NT-3 gene therapy.

One-µm thick plastic sections from an untreated mouse (A) showing subpial axon loss (asterisk) contrasting with a sample from NT-3 treated cohort (B), revealing preservation of the white matter long tracts in the descending anterolateral corticospinal tract at 7 weeks post treatment. Higher magnification image from an untreated mouse (C) showing subpial inflammation (star) and thinly remyelinated axons (arrows) which are in abundance in the NT-3 treated sample (D). Subpial area from ventral spinal cord of wild type (WT) is included for comparison (E). Scale bars are 50 μm for A and B, 10 μm for C, D and E.