Fig. 3: AAV6 transduction in healthy and IL-13 treated ALI cultures in vitro.
From: AAV-mediated MUC5AC siRNA delivery to prevent mucociliary dysfunction in asthma
A Representative images showing AAV6-eGFP transduction in undifferentiated BCi-NS1.1 cultures at MOI of 103, 104, and 105. Scale bar = 50 µm. B Representative images showing AAV6-eGFP transduction in IL-13 treated ALI cultures with respect to untransduced IL-13 treated controls. Scale bar = 20 µm. C Bar graphs showing GFP integrated density quantified from immunofluorescence images (B) in IL-13 treated AAV6-eGFP transduced ALI cultures with respect to untransduced IL-13 treated controls (n = 9). ****p < 0.0001 by Welch’s t test. Each dot represents different images.
