Fig. 5: Mucociliary transport is maintained in MUC5AC siRNA treated HAE cultures challenged with IL-13.

A Schematic showing the design of MCT measurement. Briefly, 2 µm beads are applied onto mucus on ALI cultures. The coordinated beating of cilia causes the mucus with beads to flow, which is imaged. B Representative trajectories of single beads in untreated control, IL-13 treated control, and AAV6-5AC siRNA treated group. Dark blue represents trajectories at 0 s and dark red represents trajectories at 10 s. C Scatter plot represents median velocities (µm/s) of beads in each video for untreated control, IL-13 treated control, and AAV6-5AC siRNA groups (n = 30). **p < 0.01 by Kruskal–Wallis test with Dunn’s correction. D Box and Whisker plot representing velocities (µm/s) of all beads in untreated control, IL-13 treated control and AAV6-5AC siRNA groups. Shaded region indicates particles with velocities <0.1 µm/s. ****p < 0.0001 by Kruskal–Wallis test with Dunn’s correction. E Scatter plot showing immobile fraction (no. of beads with velocity <0.1 µm/s/ total beads) in each video for untreated control, IL-13 treated control and AAV6-5AC siRNA groups. ***p < 0.001 by Kruskal–Wallis test with Dunn’s correction. F Bar graph showing ciliary beat frequency (Hz) in untreated control, IL-13 treated control, and AAV6-5AC siRNA groups.