Table 1 Antibodies used for flow cytometry.
From: AAV-mediated MUC5AC siRNA delivery to prevent mucociliary dysfunction in asthma
Cell marker | Primary/conjugated antibody | Secondary antibody | Manufacturer |
|---|---|---|---|
Basal cells (CD271) | AlexaFluor 488 conjugated | Â | eBioscience (#53-9400-42) |
Ciliated cells (α-Tubulin) | AlexaFluor 488 conjugated | – | Biolegend (#627906) |
Goblet cells (MUC5AC) | Mouse anti-MUC5AC | Anti-mouse AlexaFluor488 | ThermoFisher, Biolegend (MA5-12178, 406001) |
Secretory cells (TSPAN8) | AlexaFluor 488 conjugated | – | FisherScientific (FAB6524G100) |
