Fig. 2: Experimental setup of our in vitro esophageal tumor model inside a tissue culture CO2 incubator. | Gene Therapy

Fig. 2: Experimental setup of our in vitro esophageal tumor model inside a tissue culture CO2 incubator.

From: Drinkable gene therapy foam for the treatment of constrictive esophageal carcinoma

Fig. 2

a Photo of the setup, which includes a syringe pump to pump artificial saliva into the model esophagus, a tube lined with a monolayer of mucus secreting epithelial cells, and a tumorous obstruction in the distal part of the esophagus tubing. b Close-up photo of the esophageal tube. c Light microscopy image of HT29-MTX-E12 cells lining the inner wall of the tube. Cells were stained with Alcian Blue to visualize mucus. Scale bar: 50 μm. d Light microscopy image of OACM5.1 C esophageal carcinoma cells in three-dimensional collagen plugs. Cross sections were stained with Neutral Red to better visualize cells. Scale bar: 25 μm. e Close-up photo of the esophageal tube with inserted tumor plugs from below.

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