Fig. 2: Macrophages are predominant classical HLA Class II positive cells in the TME of high-risk neuroblastoma.
From: Macrophage-mediated anti-tumor immunity against high-risk neuroblastoma
Macrophage signature genes (CD14, CD68, and CD163) were expressed at significantly higher levels compared to dendritic cell signature genes (CD1C, CLEC9A, and LAMP3) and B cell signature genes (CD19, CD20, and CD79A). All three genes in each cell type exhibited similar expression levels. The expression of HLA-DRA in high-risk neuroblastoma tissues was shown as a representative of the overall antigen-presenting cell population. Similar data were also obtained for Cohort 2 (see Fig. S2). To maximize accuracy of the analysis, we used three signature genes for a given cell type to evaluate relative abundance of each cell type. The expression unit of genes in Cohort 1 is Reads Per Million (RPM). Each number in the figure panels indicates the average expression level of the gene indicated. The horizontal bars at 2 Log2 RPM were included as a reference for the expression level. Statistical analysis was done using a two-tailed Student’s t test.
