Fig. 3: Analysis of the change in gene expression in response to RALY-OE. | Genes & Immunity

Fig. 3: Analysis of the change in gene expression in response to RALY-OE.

From: RALY regulate the proliferation and expression of immune/inflammatory response genes via alternative splicing of FOS

Fig. 3

A RALY expression quantified by RNA-seq data. FPKM values were calculated as that has been explained in Materials and Methods. B Heat map shows the hierarchically clustered Person’s correlation matrix resulting from comparing the transcript expression values for control and RALY-OE values for control and RALY-OE samples. C Identification of RALY-regulated genes. Up-regulated genes are labeled in red, whereas down-regulated are labeled in blue in the volcano plot. D The top 10 representative GO biological processes of upregulated genes. E The top 10 representative GO biological processes of down-regulated genes. F The top 10 representative KEGG pathways of up-regulated genes. G The top 10 representative KEGG pathways of up-regulated genes. H Hierarchical clustering of DEGs in control and RALY overexpression samples. FPKM values are log2-transformed and then median-centered by each gene. Error bars represent mean ± SEM. ***p < 0.001.

Back to article page