Fig. 6: Validation of RALY regulated splicing events.

A classification of different AS types regulated by the RALY. B overlap analysis between RALY regulated genes and splicing genes. C The Top 10 GO biological process analysis. D The Top 10 KEGG functional pathway of splicing gene. E ASEs in HNRNPUL. F ASEs in GABPB1. G ASEs in HMGB1. The IGV-sashimi plots showing the alternative splicing changes that occurred in the control or RALY overexpression HeLa cells. The results for ZNF638, HNRNPUL1, GABPB1, HMGB1, CTNND1, E4F1, ZSCAN32, SPOCD1, VEZF1, and ETV1 are presented. RNA-seq quantification and RT-PCR validation of alternative splicing regulation are shown at the bottom. *P < 0.05, **P < 0.01.