Fig. 3: Decrease in capsaicin sensitivity and increase in isoflurane sensitivity in mutants TRPV1 (T612M; N394del) transfected in FDB muscles fibers of trpv1−/− mice and effect of Trpv1 inhibition by capsazepine on heat-induced sudden death in Y524S mice heat challenged at 37 °C. | Genetics in Medicine

Fig. 3: Decrease in capsaicin sensitivity and increase in isoflurane sensitivity in mutants TRPV1 (T612M; N394del) transfected in FDB muscles fibers of trpv1−/− mice and effect of Trpv1 inhibition by capsazepine on heat-induced sudden death in Y524S mice heat challenged at 37 °C.

From: TRPV1 variants impair intracellular Ca2+ signaling and may confer susceptibility to malignant hyperthermia

Fig. 3

Changes in fluorescence ratio F/F0 (peak-resting) induced by (a,b) (100 μM) capsaicin or (c,d) isoflurane (6 mM) in C57Bl6J (black), TRPV1−/− (red), humanTRPV1-mcherry (gray), TRPV1 T612M-mcherry (blue), or TRPV1 N394del-mcherry (green). Corresponding scatterplots of max value expressed as median. For the capsaicin response, data are from 42 cells (WT), Eleven cells (hTRPV1), 12 cells (T612M), 10 cells (N394del), and 9 cells (TRPV1−/−) from at least 4 independent fiber preparations. For the isoflurane response, data are from 29 cells (WT), 18 cells (hTRPV1), 11 cells (T612M), 12 cells (N394del), and 13 cells (TRPV1−/−) from at least 4 independent fiber preparations. Kruskal–Wallis tests with Dunn's post hoc tests were performed: *p < 0.05, **p < 0.001, ***p < 0.0001, ****p < 0.0001. (e) Oxygen consumption (VO2) during a 15 min exposure of mice at 37 °C in Y524S RYR1 mouse model (YS) or in WT mouse (WT). n = 8 for each condition

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