Fig. 1
From: A functional assay–based procedure to classify mismatch repair gene variants in Lynch syndrome
Outline of the cell-free in vitro MMR activity (CIMRA) assay and of this study. (a) Outline of the CIMRA assay. The figure describes testing of an MSH2 variant but testing MLH1 variants is technically similar. (b) Flow diagram for major steps of the study design. The variants in the "CIMRA Assays set 1 and 2" boxes are coloured according to their InSiGHT classification, see also Fig. 2. PCR polymerase chain reaction, ROC receiver operating characteristic, WT wild type.
