Fig. 5 | Genetics in Medicine

Fig. 5

From: The CHD4-related syndrome: a comprehensive investigation of the clinical spectrum, genotype–phenotype correlations, and molecular basis

Fig. 5

Chromatin remodeling assay. (a) Remodeling activity of CHD4 wild-type (WT) and mutants was assessed and quantified. 12, 6, and 3 nM enzyme was incubated with 12 nM recombinant nucleosome in the presence of HhaI. Digested DNA fragments were analyzed on 6% native polyacrylamide gel. (b) Quantitation of remodeling activity. Experimental data are presented as means with standard deviation. Three individual experiments from two individual purifications (N = 6) were performed. (c) A two-way analysis of variance (ANOVA) with Dunnett’s multiple test correction was performed to assess the significance of observed differences in activity. Table shows the significant P values for each variant and at each dose. Ns not significant.

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