Fig. 2: Enzyme activity and protein expression of LARS1 at 37 °C and 40 °C. | Genetics in Medicine

Fig. 2: Enzyme activity and protein expression of LARS1 at 37 °C and 40 °C.

From: Genotypic diversity and phenotypic spectrum of infantile liver failure syndrome type 1 due to variants in LARS1

Fig. 2

(a) Aminoacylation measured in fibroblasts of patients and controls cultured at 37 °C or 40 °C for 48 hours. Error bars indicate standard deviations. Enzyme activity measured at 40 °C differed significantly compared with controls (p values Table S3) in all patient samples. Comparison of 40 °C aminoacylation with the activity at 37 °C in the same sample showed significant decrease upon temperature shift in three patient cells (see asterisks). (b) LARS1 protein expression by western blotting in fibroblasts of patients and controls cultured at 37 °C or 40 °C during 48 hours.

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