Fig. 2

Phylogenetic analysis and genetic effects of positive selection loci of ZmFKF1b among maize germplasms. a The phylogenetic tree of ZmFKF1b gene inferred from 87 maize inbred lines. Support values of the main branches are shown for nodes as neighbor-joining bootstrap/maximum parsimony bootstrap/maximum likelihood bootstrap/Bayesian inference posterior probability. b Residues under positive selection falling on the protein surface. Structures of a random coil in the N-terminal, LOV, F-box, and Kelch repeat domains are shown in red, orange, green, and light blue, respectively. Eight amino acid sites (T40, D58, Q122, I184, M207, D616, R617, and Y618) undergoing positive selection (blue balls) were mapped onto the outer surface of the protein structure. c Estimated effects of haplotypes consisting of eight positive selection loci on DFF among the 87 maize inbred lines. DMF, DFF, DFF I, and DFF II indicate days to male flowering and days to female flowering collected from BJ, and photoperiod sensitive index of DFF collected from long-day and short-day conditions (BJ-HN and SC-HN), respectively. BJ Beijing, HN Hainan, SC Sichuan