Table 1 Nucleotide diversity and neutrality test of ZmFKF1s in maize and teosinte

Gene	Taxon	N ^a	Region	Size(bp)^b	π ( × 10 ⁻³)	θ ( × 10 ⁻³)	K	Tajima’s D	D '	F '
ZmFKF1a	Maize	87	5′-UTR	276	6.85	8.63	1.889	−0.56	−0.97	−0.9
			Exon1	226	5.20	7.03	1.175	−0.64	−2.68*	−2.35*
			Intron	1492	15.26	23.28	22.77	−1.17	−1.89	−1.90
			Exon2	1592	5.91	4.99	9.41	0.59	0.26	0.46
			3′-UTR	351	8.97	17.53	3.15	−1.52	−2.42*	−2.48*
			Total	3940	9.73	13.30	38.32	−0.92	−1.81	−1.72
ZmFKF1b	Maize	87	5′-UTR	116	6.99	15.40	0.81	−1.39	−3.81**	−3.53**
			Exon1	181	10.53	36.19	1.91	−2.21**	−6.35**	−5.68**
			Intron	690	11.38	24.17	7.85	−1.75	−6.59**	−5.52**
			Exon2	1583	6.45	23.33	10.21	−2.46**	−8.08**	−6.85**
			3’-UTR	382	14.21	23.91	5.43	−1.3	−5.42**	−4.54**
			Total	2952	8.88	24.07	26.21	−2.16*	−7.83**	−6.49**
ZmFKF1b	Teosinte	33	5′-UTR	306	15.94	30.60	4.88	−1.73	−2.86*	−2.94*
			Exon1	250	6.11	14.78	1.53	−1.94*	−2.92*	−3.07*
			Intron	659	19.01	30.66	12.53	−1.42	−1.31	−1.60
			Exon2	1591	7.86	19.82	12.50	−2.29**	−4.21**	−4.21**
			3′-UTR	481	13.92	28.69	6.70	−1.90*	−1.94	−2.28
			Total	3287	11.6	23.9	38.13	−1.97*	−3.01*	−3.15*

π: Kimura’s nucleotide diversity per base pair estimated from the average heterozygosity per site
θ: Watterson’s nucleotide diversity per base pair estimated from the number of segregating sites
K: average number of nucleotide differences. Dʹ: Fu and Li’s D; Fʹ: Fu and Li’s F
^aThe number of analyzed maize and teosinte lines
^bThe total number of sites (excluding sites with gaps/missing data)
*P < 0.05, **P < 0.01

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