Fig. 2

The global pattern of Populus DNA methylomes under well-watered conditions. a Distribution of CG, CHG, and CHH methylation levels (mC/total C×100%) in each sequence context of gene-related regions, including upstream, UTR, exon, intron, and downstream. Error bars indicate 95% confidence intervals generated by 1000 bootstrap replicates. b–f The average methylation level at CNS sites and their flanking regions in distinct sequence contexts. g The average methylation level of random selected regions. Average DNA methylation levels in each CNS region were split into four bins and their flanking regions were divided into 12 equally sized bins of 100 bp. Purple, green, and red indicate the distribution of CpG, CHG, and CHH methylation levels, respectively. The methylation data from the previous study (Liang et al. 2014) were reanalyzed for this experiment