Table 2 Analyses of the molecular variance (AMOVA) in A. lixula

Source of variation	d.f.	Fixation index	% variation	P value	Grouping
Brazil, Macaronesia, Mediterranean
Among group (3 groups, 13 populations)	2	F_CT: 0.033	3.30	0.000 **	1. Ita, Cfr 2. Fai, Gig, Cav 3. Ben, Col, Pop, Sic, Cre, Kos, Tor, Her
Among populations within groups	10	F_SC: 0.008	0.80	0.000 **
Within populations	298	F_IS: 0.306	29.32	0.000 **
Within individuals	302	F_IT: 0.334	66.58	0.000 **
Total	603
Macaronesia−Mediterranean
Among group (2 groups, 11 populations)	1	F_CT: 0.013	1.30	0.006 **	1. Fai, Gig, Cav 2. Ben, Col, Pop, Sic, Cre, Kos, Tor, Her
Among populations within groups	9	F_SC: 0.009	0.87	0.000 **
Within populations	253	F_IS: 0.309	30.32	0.000 **
Within individuals	264	F_IT: 0.325	67.51	0.000 **
Total	527
Mediterranean−Alboran Sea
Among group (2 groups, 8 populations)	1	F_CT: 0.005	0.54	0.088	1. Ben, Col, Pop, Sic, Cre, Kos 2. Tor, Her
Among populations within groups	6	F_SC: 0.006	0.60	0.002 **
Within populations	184	F_IS: 0.320	31.61	0.000 **
Within individuals	192	F_IT: 0.327	67.26	0.000 **
Total	383
Western Mediterranean−Eastern Mediterranean
Among group (2 groups, 6 populations)	1	F_CT: 0.001	0.13	0.393	1. Ben, Col, Pop 2. Sic, Cre, Kos
Among populations within groups	4	F_SC: 0.001	0.03	0.472
Within populations	138	F_IS: 0.326	32.58	0.000 **
Within individuals	144	F_IT: 0.327	67.26	0.000 **
Total	287

Four different groupings and subsets of populations are represented. Populations comprising each group are listed on the right column
* Significant at p < 0.05; ** Significant at p < 0.01

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