Fig. 1
From: Unraveling the causes of adaptive benefits of synonymous mutations in TEM-1 β-lactamase
An overview of the panel of synonymous and non-synonymous mutations. For all panels, blue data correspond to the synonymous mutations and red to the non-synonymous mutations. In a, resistance against CTX in μg/mL [IC99.99] is given for the whole panel of TEM alleles. Error bars are standard errors of the mean (N = 3). In b, the location of the mutations and key features of TEM are given, with numbers indicating amino-acid positions. In c, the mutations present in the mature TEM protein are shown (four synonymous mutations occur in the signal peptide), with numbers indicating amino-acid positions. In d and e, the codon usage for original TEM sequence (x-axis) and the mutants (y-axis) is given, with units codons per 1000 codon positions. d Codon use for the non-synonymous mutations, and e codon use for the synonymous mutations. Although some mutations lead to clear differences in codon usage, there is no systematic pattern for either mutation class
