Fig. 2

Linear regression analysis of standardized, phylogenetically independent contrasts (PICs) for all pairings of latitude-separated sister lineages belonging to Arthropoda (n = 7900), whereby each point represents one sister pair and the fitted regression line is forced through the origin. (a) PICs in median latitude vs. signed PICs in branch length (positive sign = larger branch length at lower latitude) (slope of regression = 5.36E-06). (b) PICs in median temperature vs. signed PICs in branch length (slope of regression = 6.73E-06). Each pairing of lineages is separated by a minimum of 20 degrees in median absolute latitude and 0.02–0.15 sequence divergence. Each pairing of lineages is color/symbol coded differently according to major insect order or gray/plus symbol for remaining orders within Arthropoda using the data visualization software plotly (https://plot.ly/)