Fig. 2: Functional validation of AOP2 alleles.
From: Revisiting a GWAS peak in Arabidopsis thaliana reveals possible confounding by genetic heterogeneity
A The gene model of AOP2.1. Colored parts are conserved protein domains. Triangles show predicted indels that could cause frameshift. + and − are the indel size from the Col-0 reference sequence. B Accumulation of 2-propenyl glucosinolate in each type of allele and the AOP2 expression. Marks from a to e are corresponding to the indels. C The flowering time based on AOP2 indel genotypes. “Functional AOP2” corresponds to lines without frameshift (n = 86) and “truncated AOP2“ corresponds to lines with frameshift (a–d; n = 19, Table S1). To control for the effect of chr4:1355057 (which is known to be associated with flowering time variation), only chr4:1355057 reference lines with expressed AOP2 were used (p = 0.07 by two-tailed Welch’s t-test).
