Fig. 5: Phylogenetic tree showing relationships among AGH sequences from terrestrial isopods.

AGH2 and AGHY3 are missing from this phylogeny because those sequences were omitted because of their short length. The tree was generated using all AGH-like sequences from T. rathkei of at least 100 bp, along with reference AGH nucleotide sequences from other species, aligning them with MUSCLE v. 3.8.31 (Edgar 2004), selecting a model using ModelTest-NG v. 0.1.6 (Darriba et al. 2020), and running the analysis in RAxML-NG v. 0.9.0 (Stamatakis 2014) with 100 bootstrap replicates.