Fig. 5: Bulk-segregant analysis (BSA) for comparing allele frequencies from ‘low’ and ‘high’ tuber sprouting bulks designed using t46 time-point trait.

a Chromosomes, b gene density, c BSA: low sprout-growth rate bulk (observed) versus high sprout-growth rate bulk (expected); d BSA: high sprout-growth rate bulk (observed) versus low sprout-growth rate bulk (expected); e SNP density; f QTL detected by composite interval mapping (CIM) using t46 trait data. The terms ‘observed’ and ‘expected’ in (c) and (d) above refer to the assumptions set for using allele frequencies for performing the Chi-squared tests in reciprocal BSA analyses. Red and blue lines represent relative skewness (RS) measures while grey lines illustrate average-Q-values (AQV) estimates observed in the two reciprocal BSA comparisons for assessing non-random distribution of allele frequencies between the two bulks.