Table 1 Screening of natural population of Ligustrum vulgare for incompatibility groups using stigma tests.

Population name	Population identifier	Region	Country	GPS coordinates		Altitude (m)	Year of sampling	G_A individuals				G_B individuals				G_N individuals	SI (G_A + G_B)	SC (G_A + G_B)	Estimated frequencies (95% confidence interval)
Population name	Population identifier	Region	Country	GPS coordinates		Altitude (m)	Year of sampling	Total	SI	SC	nt	Total	SI	SC	nt		SI (G_A + G_B)	SC (G_A + G_B)	G_A	G_B	SC
Canche^a	CA	North sea	France	N 50° 31′ 25.813	E 01°37′ 32.311	30	2012–2017	21	17	1	3	28	23	0	5	0	40	1	0.41 (0.27–0.56)	0.56 (0.41–0.71)	0.02 (0.0–0.17)
Audresselles^a	Au	North sea	France	N 50° 49′ 10.662	E 01°35′ 44.796	10	2013	9	7	1	1	11	10	0	1	0	17	1	0.39 (0.22–0.65)	0.56 (0.39–0.82)	0.06 (0.0–0.32)
Platier d’Oye^a	PO	North sea	France	N 50° 59′ 50.118	E 02° 02′ 31.984	0	2013	9	9	0	0	8	7	0	1	0	16	0	0.56 (0.37–0.83)	0.44 (0.25–0.71)	0.0 (0.0–0.27)
Montjardin^a	MJ	Cévennes	France	N 44° 07′ 48.089	E 03° 24′ 10.226	1000	2015–2017	24	14	3	7	20	13	0	7	0	27	3	0.47 (0.3–0.66)	0.43 (0.27–0.62)	0.1 (0.0–0.29)
Vif^a	VIF	Alpes	France	N 45° 00′ 23.965	E 05° 40′ 46.549	515	2015	7	2	1	4	3	0	0	3	0	2	1	0.67 (0.33–1.0)	0.0 (0.0–0.35)	0.33 (0.0–0.69)
Isar^b	G	Bavaria	Germany	N 48° 25′ 27.181	E 11° 53′ 26.653	450	2015	9	7	1	1	13	10	0	3	0	17	1	0.39 (0.22–0.65)	0.56 (0.39–0.82)	0.06 (0.0–0.32)
San Mariano	SM	Umbria	Italy	N 43° 04′ 54.625	E 12° 18′ 28.759	300	2014	15	9	0	6	7	7	0	0	0	16	0	0.56 (0.37–0.83)	0.44 (0.25–0.71)	0.0 (0.0–0.27)
Total								94	65	7	22	90	70	0	20	0	135	7	0.46 (0.38–0.55)	0.49 (0.41–0.58)	0.05 (0.0–0.14)

Sampled individuals were tested as pollen recipients using the pollen from two inter-compatible tester plants and, when enough flowers were available, using self-pollen (nt: individuals for which self-pollen was not tested). G_A: individuals incompatible with tester A and compatible with tester B. G_B: individuals compatible with tester A and incompatible with tester B. G_N: individuals compatible with both tester A and tester B. SI: individuals for which self-pollen did not germinate on their own stigmas. SC: individuals for which self-pollen germinated on their own stigmas. The frequencies given in the three last columns were calculated on plants assessed both for SI group (using pollen from tester A and B) and self-pollen.
^aPopulations from which cuttings of individual genotypes were transferred to the experimental garden.
^bPopulation from which collected seeds were sown and grown in the experimental garden.

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