Fig. 6: Bars represent the proportion of EMS-induced mutations at each trinucleotide motif centered at mutated sites (5ʹ-3ʹ orientation), whereas the lines represent the observed proportion of trinucleotide frequencies observed in the Daphnia reference assemblies.

NAN and NTN trinucleotides are significantly underrepresented, whereas many of the NGN and NCN motifs are overrepresented (indicated by asterisks).