Table 1 Summary of mutations induced by 10 and 25 mM EMS.
EMS concentration | Daphnia species | Average number of mutations (SD) | Base substitution mutation rate (SEM) | Average number of mutations in genes (SD) | Per gene mutation rate (SEM) | Average number of non-synonymous mutations (SD) | Non-synonymous mutation rate (SEM) |
|---|---|---|---|---|---|---|---|
10 mM | D. pulex (OP) | 126 (0.71) | 1.24 × 10−6 (3.09 × 10−7) | 48 (2) | 3.9 × 10−3 (1.2 × 10−3) | 21 (0) | 1.9 × 10−3 (4.6 × 10−4) |
D. pulex (CP) | 124 (32) | 1.32 × 10−6 (8.47 × 10−9) | 58 (18) | 2.4 × 10−3 (1.3 × 10−4) | 28 (5) | 1.0 × 10−3 (3.4 × 10−6) | |
D. pulicaria | 94 (22) | 9.40 × 10−7 (2.35 × 10−7) | 40 (60) | 1.9 × 10−3 (2.7 × 10−4) | 16 (5) | 7.8 × 10−4 (2.3 × 10−4) | |
25 mM | D. pulex (OP) | 154 (43) | 1.69 × 10−6 (1.0 × 10−6) | 65 (16) | 4.1 × 10−3 (2.1 × 10−3) | 36 (8) | 1.8 × 10−3 (8.7 × 10−4) |
D. pulex (CP) | 171 (104) | 1.58 × 10−6 (4.34 × 10−7) | 78 (40) | 3.2 × 10−3 (7.5 × 10−4) | 30 (14) | 1.7 × 10−3 (3.7 × 10−4) | |
D. pulicaria | 201 (67) | 1.98 × 10−6 (6.12 × 10−7) | 91 (27) | 4.4 × 10−3 (1.3 × 10−3) | 43 (13) | 2.1 × 10−3 (6.1 × 10−4) |
