Table 1 Sequencing details of parental line and bulks and mapping of sequence reads.

From: QTL-seq for the identification of candidate genes for days to flowering and leaf shape in pigeonpea

Sample

Number of lines bulked

Total reads generated (Million reads)

High quality reads (Million reads)

Reads mapped (Million reads)

Genome coverage at 1X

Average depth (X)

ICP 5529a

 

58.63

35.33

15.33

90.70%

9.15

EFb

15

66.34

62.64

30.28

93.13%

15.10

LFb

15

76.46

63.99

30.25

93.26%

15.08

OLSb

15

60.86

51.13

26.86

92.93%

13.39

LLSb

15

61.88

52.23

26.90

92.95%

13.41

  1. EF Early flowering pool, LF Late flowering pool, OLS Obcordate leaf shape pool, LLS Lanceolate leaf shape pool.
  2. aICP 5529 short reads were aligned to the publicly available pigeonpea genome of Asha (Varshney et al. 2012).
  3. bThe short reads of bulks were aligned to the ICP 5529 assembly developed by replacement of SNPs between ICP 5529 and Asha.
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