Table 2 Identification of SNPs in putative candidate genes for days to flowering.

From: QTL-seq for the identification of candidate genes for days to flowering and leaf shape in pigeonpea

Linkage group

Gene

Position

ICP 5529 allele

LF allele

SNP index (LF pool)a

EF allele

SNP index (EF pool)b

Δ SNP-indexc

SNP effect

Function

CcLG03

C.cajan_09900

19222701

G (aCg)d

G (aCg)d

0

A (aTg)d

1

−1

Exon (nsSNP)

Pentatricopeptide repeat-containing protein

CcLG03

C.cajan_09938

19549631

A

A

0

G

1

−1

Intron

Chromodomain-helicase-DNA-binding protein 4

CcLG03

C.cajan_09958

19690312

G

G

0

A

1

−1

Intron

Maestro heat-like repeat-containing protein family

CcLG03

C.cajan_09965

19763754

C

C

0

T

1

−1

Intron

Phosphatidylinositol 4-phosphate 5-kinase 9

CcLG03

C.cajan_10046

20439904

G

G

0

C

1

−1

Intron

1,4-alpha-glucan-branching enzyme

CcLG03

C.cajan_10067

20635496

T

T

0

A

1

−1

Intron

Uridine nucleosidase 1

CcLG03

C.cajan_10078

20745506

G

G

0

T

1

−1

Exon (sSNP)

Cell division protein

CcLG03

C.cajan_10078

20745771

A

A

0

G

1

−1

Intron

 

CcLG03

C.cajan_10078

20747419

C

C

0

T

1

−1

Intron

 
  1. nsSNP non-synonymous SNPS, sSNP synonymous SNP.
  2. aSNP-index of late flowering (LF) was calculated based on the allele calls and read depth in comparison to ICP 5529 reference assembly.
  3. bSNP-index of early flowering (EF) bulk was calculated based on the allele calls and read depth in comparison to ICP 5529 reference assembly.
  4. cΔ SNP-index of each SNP positions was calculated using following formula: Δ SNP-index = SNP-index of LF − SNP-index of EF.
  5. dValue in parenthesis indicates the codon change due to SNP/Code for changed amino acids.