Table 3 Identification of SNPs in putative candidate genes for leaf shape.

From: QTL-seq for the identification of candidate genes for days to flowering and leaf shape in pigeonpea

Linkage group

Gene

Position

ICP 5529 allele

OLS allele

SNP index (OLS pool)a

LLS allele

SNP index (LLS Pool)b

Δ SNP-indexc

SNP Effect

Function

CcLG08

C.cajan_15985

6701814

A

A

0

T

1

−1

Intron

Beta-carotene hydroxylase 2

CcLG08

C.cajan_15991

6764651

T (Tgt)d

T (Tgt)d

0

C (Cgt)d

1

−1

Exon (nsSNP)

Ac-like transposase

CcLG08

C.cajan_16002

6915910

T (aTg)d

T (aTg)d

0

C (aCg)d

1

−1

Exon (nsSNP)

Uncharacterized protein

CcLG08

C.cajan_16003

6921340

A

A

0

G

1

−1

Exon (sSNP)

Pro-Pol polyprotein

CcLG08

C.cajan_16003

6923929

T

T

0

C

1

−1

Intron

 

CcLG08

C.cajan_16003

6924854

A

A

0

G

1

−1

Intron

 

CcLG08

C.cajan_16003

6927480

C

C

0

T

1

−1

Intron

 

CcLG08

C.cajan_16003

6927533

A

A

0

G

1

−1

Intron

 

CcLG08

C.cajan_16003

6927560

T

T

0

C

1

−1

Intron

 

CcLG08

C.cajan_16003

6927678

T

T

0

C

1

−1

Intron

 

CcLG08

C.cajan_16012

7057478

G (Gca)d

G (Gca)d

0

A (Aca)d

1

−1

Exon (nsSNP)

F-box protein

CcLG08

C.cajan_16012

7059171

T

T

0

C

1

−1

Intron

 

CcLG08

C.cajan_16013

7068488

G

G

0

C

1

−1

Intron

Uncharacterized protein

CcLG08

C.cajan_16013

7068679

G

G

0

A

1

Intron

 

CcLG08

C.cajan_16013

7070780

C (cCt)d

C (cCt)d

0

T (cTt)d

1

−1

Exon (nsSNP)

 

CcLG08

C.cajan_16014

7083922

T

T

0

A

1

−1

Intron

Transcriptional corepressor

CcLG08

C.cajan_16014

7093751

G

G

0

A

1

−1

Intron

 

CcLG08

C.cajan_16038

7456634

T

T

0

C

1

−1

Intron

Cytochrome P450

CcLG08

C.cajan_16038

7456764

A

A

0

G

1

−1

Intron

 

CcLG08

C.cajan_16038

7456831

T

T

0

A

1

−1

Intron

 

CcLG08

C.cajan_16038

7456974

A

A

0

G

1

−1

Intron

 

CcLG08

C.cajan_16038

7457844

C

C

0

A

1

−1

Intron

 

CcLG08

C.cajan_16041

7486941

A

A

0

G

1

−1

Intron

Uncharacterized protein

CcLG08

C.cajan_16047

7606346

T

T

0

A

1

−1

Intron

Transposon Ty3-I

CcLG08

C.cajan_16049

7641790

A

A

0

C

1

−1

Intron

E3 ubiquitin-protein ligase

CcLG08

C.cajan_16049

7642733

G

G

0

A

1

−1

Intron

 

CcLG08

C.cajan_16049

7643315

A

A

0

C

1

−1

Exon (sSNP)

 

CcLG08

C.cajan_16051

7666784

A

A

0

G

1

−1

Intron

Protein ROOT PRIMORDIUM DEFECTIVE 1

CcLG08

C.cajan_16051

7667174

T

T

0

C

1

−1

Intron

 

CcLG08

C.cajan_16059

7780600

A

A

0

T

1

−1

Intron

Probable methyltransferase PMT16

CcLG08

C.cajan_16061

7838639

C

C

0

T

1

−1

Exon (sSNP)

1-aminocyclopropane-1-carboxylate oxidase homolog 1

CcLG08

C.cajan_16062

7870949

A

A

0

G

1

−1

Intron

 

CcLG08

C.cajan_16063

7888623

G

G

0

A

1

−1

Intron

1-aminocyclopropane-1-carboxylate oxidase homolog 12

CcLG08

C.cajan_16066

7940765

C

C

0

G

1

−1

Intron

Tripeptidyl-peptidase 2

CcLG08

C.cajan_16066

7947009

G

G

0

A

1

−1

Intron

 

CcLG08

C.cajan_16066

7950673

A

A

0

C

1

−1

Intron

 

CcLG08

C.cajan_16068

8008253

C

C

0

A

1

−1

Intron

Cytochrome P450

CcLG08

C.cajan_16074

8168767

C

C

0

A

1

−1

Intron

Type I inositol

CcLG08

C.cajan_16099

8666995

T

T

0

C

1

−1

Exon (sSNP)

-

  1. nsSNP non-synonymous SNPS, sSNP synonymous SN.
  2. aSNP-index of obcordate leaf shape (OLS) bulk was calculated based on the allele calls and read depth in comparison to ICP 5529 reference assembly.
  3. bSNP-index of lanceolate leaf shape (LLS) bulk was calculated based on the allele calls and read depth in comparison to ICP 5529 reference assembly.
  4. cΔ SNP-index of each SNP positions was calculated using following formula: Δ SNP-index = SNP-index of OLS − SNP-index of LLS.
  5. dValue in parenthesis indicates the codon change due to SNP/Code for changed amino acids.