Fig. 2: Average recombination rate (cM/kb) calculated for the syntenic intervals of 4 (on ChrVI) and 13 (on ChrXI) in S. cerevisiae and S.uvarum across all measured temperatures.

S. cerevisiae recombination rate estimates were corrected for fluorescence extinction using a maximum likelihood model derived in Raffoux et al. (2018a). S. uvarum recombination rate estimates were corrected for fluorescence extinction using a derivative of this script adjusted for only two fluorescent loci. No data was collected for S. uvarum strains at 42 °C. In strain-level comparisons (A), strain name refers to the parent strain crossed with each fluorescent tester to produce a diploid for sporulation. Error bars indicate standard deviation above and below the mean, as calculated between biological replicates of each strain. In species-level comparisons, (B) error bars indicate standard error across all species’ strain replicates.