Fig. 5: Sequence of P-012.1 amplicon and the positions of detected SNPs.

Six SNPs identified at positions 28, 71, 77, 96, 130, and 149 are highlighted by black color. These six SNPs reveal six distinct haplotypes. Three of the haplotypes (P-012.1-S1, -S2, and –S3) are associated with a slow or an intermediate rate of deterioration, while the other three haplotypes (P-012.1-R1, -R2, and –R3) are associated with a rapid rate of deterioration in fresh-cut lettuce. Two SNPs at the positions 96 (P-012.1-096) and 149 (P-012.1-149) can be used to differentiate between accession with slow and rapid rates of deterioration. These two SNPs (indicated by asterisks) correspond to SNPs Lsat_1_v8_lg_4_284,212,500 and Lsat_1_v8_lg_4_284,212,553 on the physical map of lettuce genome19, version 8