Fig. 4: PCR verification of non-transgenic pds mutant lines using three primer sets (Fig. 1b) targeted to the three different regions of the T-DNA insert. | Horticulture Research

Fig. 4: PCR verification of non-transgenic pds mutant lines using three primer sets (Fig. 1b) targeted to the three different regions of the T-DNA insert.

From: A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

Fig. 4

Primer set 1 was used to amplify the T-DNA region between the left T-DNA border and the 5′ end of the CaMV 35S promoter. Primer set 2 was used to amplify the 3′ end of the Cas9 coding sequence. Primer set 3 was used to amplify a region of the kanamycin resistance gene (KanR). The absence of all three T-DNA PCR fragments was indicative of non-transgenic plants. WT wild type

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