Table 1 pds mutant tobacco shoots regenerated without kanamycin selection

From: A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

Transformation experiment

No. of explants1

Total no. of independent pds mutant shoots2

Mutation efficiency (%)3

1

159

53

33.3

2

88

59

67.1

3

168

85

50.6

Total

415

197

47.5

  1. 1The number of tobacco leaf explants infected with Agrobacterium tumefaciens harboring hCas9-NtPDS
  2. 2The total number of independent pds mutant shoots as indicated by an albino phenotype
  3. 3The mutation efficiency was calculated as follows: \((\frac {\mathrm {no}. \mathrm{of}\,pds\, \mathrm {mutant}\, \mathrm {shoots}} {\mathrm {no}. \mathrm {of}\, \mathrm {explants}}) \times 100\)
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