Table 2 Mutations in individual mutant plant lines identified through high-throughput sequencing analyses

From: A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

Mutant plant line

Sequence

Mutation description

Wild type

\(\frac{A}{{43}}\frac{A}{{44}}\frac{A}{{45}}\frac{G}{{46}}\frac{A}{{47}}\frac{T}{{48}}\frac{G}{{49}}\frac{A}{{50}}\frac{T}{{51}}\frac{G}{{52}}\frac{A}{{53}}\frac{T}{{54}}\frac{G}{{55}}\)

Not applicable

pds-9

AAAGATGA_GATG

1 bp deletion @ position 51

AAAGATGATTGATG

1 bp insertion @ position 51 or 52 (T)

AAAGAT_____TG

5 bp deletion @ positions 49–53

AAAGATG__GATG

2 bp deletion @ positions 50 and 51

pds-10

AAAGAT__TGATG

2 bp deletion @ positions 49 and 50

AAAGATGA__ATG

2 bp deletion @ positions 51 and 52

pds-11

AAAGAT__TGATG

2 bp deletion @ positions 49 and 50

AAAGATGAGTGATG

1 bp insertion @ position 51 (G)

AAAGATG__GATG

2 bp deletion @ positions 50 and 51

AAAGATGA_____

48 bp deletion @ positions 51–98

pds-12

AAAGATGA_GATG

1 bp deletion @ position 51

AA_____ATGATG

5 bp deletion @ positions 45–49

AAAGAT__TGATG

2 bp deletion @ positions 49 and 50

AAA____ATGATG

4 bp deletion @ positions 46–49

pds-13

AAAGAT_ATGATG

1 bp deletion @ position 49

AAAGATG_TGATG

1 bp deletion @ position 50

A______ATGATG

6 bp deletion @ positions 44–49

AAAGATGAATGATG

1 bp insertion @ position 50 or 51 (A)

pds-14

AAAGATGATTGATG

1 bp insertion @ positions 51 or 52 (T)

AAAGAT__TGATG

2 bp deletion @ positions 49 and 50

AAA______GATG

6 bp deletion @ positions 46–51

AAAGATGAATGATG

1 bp insertion @ position 50 or 51 (A)