Table 4 Rate of non-transgenic pds mutants among all pds mutants

From: A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

Transformation experiment

No. of independent pds mutant shoots characterized1

No. of non-transgenic pds mutant shoots identified2

% of characterized mutant shoots that were non-transgenic3

1

10

2

17.2

2

10

1

3

9

2

  1. 1A total of 29 pds mutant plants were chosen for identification of non-transgenic mutants
  2. 2The absence of the transgenes in pds mutant plants was verified by PCR and a kanamycin resistance test
  3. 3Percentage was calculated by dividing the total number of non-transgenic mutants by the total number of mutant plants analyzed and multiplying by 100
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