Fig. 1: ROS and NO imaging under MV and SNP treatments.

a DCFH-DA fluorescence of the MV-treated tissue. b DCFH-DA fluorescence of the control tissues. c DAF-2DA fluorescence of the SNP-treated tissues. d DAF-2DA fluorescence of the control tissues. A small piece of tissue after 10 h of treatment including stem and petiole bases were excised, dissected lengthwise, and incubated in DCFH-DA buffer, a ROS fluorescent probe, or NO indicator dye DAF-2DA. ROS or NO imaging was observed under confocal microscopy. Bar = 600 μm. Ax axillary bud, Pe petiole, St stem