Fig. 7 | Horticulture Research

Fig. 7

From: Reactive oxygen species and nitric oxide induce senescence of rudimentary leaves and the expression profiles of the related genes in Litchi chinensis

Fig. 7

Images of a the nuclei in the control cells, b the ROS-treated cells, c the NO-treated cells, and d cell PCD ratio of the rudimentary after ROS and NO treatments. Branches of the ‘Nuomici’ litchi trees were uniformly sprayed with water as control, 120 µM methyl viologen dichloride hydrate (MV) as ROS treatment, or 3 mM sodium nitroprusside (SNP) as NO treatment. Rudimentary leaves were collected after 0, 1, 3, 5, 7, and 9 d of treatment. Nuclei images were visualized by fluorescence microscopy. Bar = 5 μm. Cell PCD ratio was given by the expression 100× numbers of PCD nuclei/total numbers of nuclei scanned. Values are means ± SE, n = 3. Different letters indicate significant difference at P < 0.05 level among treatments at the same time according to Duncan’s multiple range test

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