Table 1 Oligonucleotide sequences of real-time PCR primers and probes used in this study
From: Molecular mechanisms underlying heat or tetracycline treatments for citrus HLB control
Name | Sequence (5’–3’) | Target gene or flanking region | Reference |
|---|---|---|---|
Prophage FP1-specific primer | |||
FP1-gp110F | GAAGTGAGACGCCAGGAAAG | Possible holin | |
FP1-gp110R | TCGTACATGCACCCCTGATA | ||
FP1-gp235F | AAGGGGTCTAATCTACCGCC | Putative phage-related protein | |
FP1-gp235R | TTTGTATGGCTTGCCTCAAC | ||
Prophage FP2-specific primer | |||
FP2-gp240F | GCACGCATGGAGAGAGATTT | Putative trimeric autotransporter adhesin | |
FP2-gp240R | CCAGTCAAACCCTTTAGCCA | ||
FP2-gp065F | AATGTTTCCAAATCCGCAAG | Possible integrase | |
FP2-gp065R | GTATCGCAAGCTCAAGCACA | ||
Primers for both FP1 and FP2 | |||
gp-025F | TATGAGGAGCTCTGGGGCTA | Putative phage tail fiber protein; lectin-binding | This study |
gp-025R | TGATGTCGATGGGTTAAACG | ||
gp-030F | CAAACTCCCGTTTCACACCT | Putative phage structural protein | This study |
gp-030R | CGCTTTCCCGTTCTGAAATA | ||
gp-035F | TCAAAGATGCGGTAATGCTG | Possible endolysin | This study |
gp-035R | GGGCAGTGTTGAATGTTCCT | ||
gp-200F | CCTCTTCCGATAACGACGAA | Putative Bro-N family phage antirepressor | This study |
gp-200R | AGCCACCTGAGACCTTGCTA | ||
Pro-R.O. F | CGCACGAGGTGTAGCTTATG | rep_origin in UF506 | This study |
Pro-R.O. R | GAGGACAGCCGACGATTACT | ||
Primers for Las | |||
Las-R.O. F | TTACCAATGCGGATGGTTCT | Las oriC region in psy62 | This study |
Las-R.O. R | ATCCGAATCCCTGTTGTGAT | ||
HLBasfa | TCGAGCGCGTATGCGAATACG | 16S rDNA | |
HLBr | GCGTTATCCCGTAGAAAAAGGTAG | ||
HLBp | bAGACGGGTGAGTAACGCGc | ||
