Fig. 2: Fluorescent stereomicroscope photographs of Actinidia chinensis var. deliciosa flowers contaminated by Pseudomonas syringae pv. actinidiae (Psa) expressing a green fluorescent protein (CFBP7286-GFPuv). | Horticulture Research

Fig. 2: Fluorescent stereomicroscope photographs of Actinidia chinensis var. deliciosa flowers contaminated by Pseudomonas syringae pv. actinidiae (Psa) expressing a green fluorescent protein (CFBP7286-GFPuv).

From: Pathways of flower infection and pollen-mediated dispersion of Pseudomonas syringae pv. actinidiae, the causal agent of kiwifruit bacterial canker

Fig. 2

Inoculation was performed by pollinating stigmas of each flower with pollen contaminated by GFPuv-Psa. Bright green fluorescent emission is due to Psa CFBP7286-GFPuv colonization of tissues. Photographs were taken 24 h after inoculation (a, b, c) or 6–9 days after inoculation (d, e, f). a Stigma coated by contaminated pollen grains. b, c Psa CFBP7286-GFPuv migration along the whole stylar furrow to the ovarium (d–e). d Healthy ovarium. e Infected ovarium. The pathogen reached the ovarium via the styles (st). Central placenta vascular bundle (cb), ovary wall vascular bundle (ob) and ovules (o) are visible. Fluorescence is associated to the vascular bundles. f Flower pedicel, showing the high contamination by CFBP7286-GFPuv leading to a systemic invasion of from the ovarium into the flower-bearing cane. Bar measure is reported in each panel

Back to article page