Fig. 4: Activities of key enzymes involved in sugar metabolism in the fruit of the untransformed control (CK) and transgenic lines (A27 and A04) of “Greensleeves” apple during fruit development.

SDH sorbitol dehydrogenase, CWINV cell wall invertase, NINV neutral invertase, vAINV vacuolar acid invertase, SUSY sucrose synthase, FK fructokinase, HK hexokinase, SPS sucrose phosphate synthase. Values are means of five replicates ± SD. An asterisk indicates a significant difference between transgenic lines (A27 or A04) and CK at P < 0.05 by Tukey’s test after analysis of variance