Fig. 6: Relative mRNA expression levels of genes encoding sugar transporters in the fruit of the untransformed control (CK) and transgenic lines (A27 and A04) of “Greensleeves” apple during fruit development.

MdSOT sorbitol transporter, MdSUC sucrose carrier/transporter, MdTMT tonoplast monosaccharide transporter, MdvGT vacuolar glucose transporter. Quantitative RT-PCR was performed with gene-specific primers. For each sample, transcript levels were normalized with those of Actin, and the relative expression level for each gene was obtained via the ddCT method. Expression in 40-DAB fruit was designated as “10”. Values are means of three replicates ± SD. An asterisk indicates a significant difference between transgenic lines (A27 or A04) and CK at P < 0.05 by Tukey’s test after analysis of variance